| Primer | Target gene | Coverage | Sequence | Reference | ||
|---|---|---|---|---|---|---|
| Bacteria | Archaea | Eukaryote | ||||
| 341F | 16S | Yes | No | Low | CCTACGGGNGGCWGCAG | Herlemann 2011 |
| 341'F | 16S | Yes | Yes | Low | CCTAHGGGRBGCAGCAG | Hugerth 2014a |
| 515'F | 16S | Yes | Yes | No | GTGBCAGCMGCCGCGGTAA | Hugerth 2014a |
| 805R | 16S | Yes | Yes | Low | GACTACHVGGGTATCTAATCC | Herlemann 2011 |
| 926'R | 16S | Yes | Yes | Yes | CCGYCWATTCMTTTRAGTTT | Adapted from Parada 2015 |
| 574*F | 18S | No | No | Yes | CGGTAAYTCCAGCTCYV | Hugerth 2014b |
| 1132R | 18S | No | No | Yes | CCGTCAATTHCTTYAART | Hugerth 2014b |
Notes:
F and R means Forward and Reverse, respectively
The names for 16S primers refer to their positions in E. coli and for 18S primers in S. cerevisiae
For other primers, we recommend the review by Klindworth et al
You can also find these primers’ taxonomic coverage among sequences in the RDP database.