diff --git a/packages/nextjs/src/config/handleRunAfterProductionCompile.ts b/packages/nextjs/src/config/handleRunAfterProductionCompile.ts
index 901ba9dffa6e..f6e5a3b21617 100644
--- a/packages/nextjs/src/config/handleRunAfterProductionCompile.ts
+++ b/packages/nextjs/src/config/handleRunAfterProductionCompile.ts
@@ -1,5 +1,7 @@
 import type { createSentryBuildPluginManager as createSentryBuildPluginManagerType } from '@sentry/bundler-plugin-core';
 import { loadModule } from '@sentry/core';
+import * as fs from 'fs';
+import * as path from 'path';
 import { getBuildPluginOptions } from './getBuildPluginOptions';
 import type { SentryBuildOptions } from './types';
 
@@ -60,4 +62,65 @@ export async function handleRunAfterProductionCompile(
     prepareArtifacts: false,
   });
   await sentryBuildPluginManager.deleteArtifacts();
+
+  // After deleting source map files in turbopack builds, strip any remaining
+  // sourceMappingURL comments from client JS files. Without this, browsers request
+  // the deleted .map files, and in Next.js 16 (turbopack) those requests fall through
+  // to the app router instead of returning 404, which can break middleware-dependent
+  // features like Clerk auth.
+  const deleteSourcemapsAfterUpload = sentryBuildOptions.sourcemaps?.deleteSourcemapsAfterUpload ?? false;
+  if (deleteSourcemapsAfterUpload && buildTool === 'turbopack') {
+    await stripSourceMappingURLComments(path.join(distDir, 'static'), sentryBuildOptions.debug);
+  }
+}
+
+const SOURCEMAPPING_URL_COMMENT_REGEX = /\n?\/\/[#@] sourceMappingURL=[^\n]+$/;
+const CSS_SOURCEMAPPING_URL_COMMENT_REGEX = /\n?\/\*[#@] sourceMappingURL=[^\n]+\*\/$/;
+
+/**
+ * Strips sourceMappingURL comments from all JS/MJS/CJS/CSS files in the given directory.
+ * This prevents browsers from requesting deleted .map files.
+ */
+export async function stripSourceMappingURLComments(staticDir: string, debug?: boolean): Promise<void> {
+  let entries: string[];
+  try {
+    entries = await fs.promises.readdir(staticDir, { recursive: true }).then(e => e.map(f => String(f)));
+  } catch {
+    // Directory may not exist (e.g., no static output)
+    return;
+  }
+
+  const filesToProcess = entries.filter(
+    f => f.endsWith('.js') || f.endsWith('.mjs') || f.endsWith('.cjs') || f.endsWith('.css'),
+  );
+
+  const results = await Promise.all(
+    filesToProcess.map(async file => {
+      const filePath = path.join(staticDir, file);
+      try {
+        const content = await fs.promises.readFile(filePath, 'utf-8');
+
+        const isCSS = file.endsWith('.css');
+        const regex = isCSS ? CSS_SOURCEMAPPING_URL_COMMENT_REGEX : SOURCEMAPPING_URL_COMMENT_REGEX;
+
+        const strippedContent = content.replace(regex, '');
+        if (strippedContent !== content) {
+          await fs.promises.writeFile(filePath, strippedContent, 'utf-8');
+          return file;
+        }
+      } catch {
+        // Skip files that can't be read/written
+      }
+      return undefined;
+    }),
+  );
+
+  const strippedCount = results.filter(Boolean).length;
+
+  if (debug && strippedCount > 0) {
+    // eslint-disable-next-line no-console
+    console.debug(
+      `[@sentry/nextjs] Stripped sourceMappingURL comments from ${String(strippedCount)} file(s) to prevent requests for deleted source maps.`,
+    );
+  }
 }
diff --git a/packages/nextjs/test/config/handleRunAfterProductionCompile.test.ts b/packages/nextjs/test/config/handleRunAfterProductionCompile.test.ts
index 2d1769986158..3d551dfb6c40 100644
--- a/packages/nextjs/test/config/handleRunAfterProductionCompile.test.ts
+++ b/packages/nextjs/test/config/handleRunAfterProductionCompile.test.ts
@@ -1,6 +1,12 @@
 import { loadModule } from '@sentry/core';
-import { beforeEach, describe, expect, it, vi } from 'vitest';
-import { handleRunAfterProductionCompile } from '../../src/config/handleRunAfterProductionCompile';
+import * as fs from 'fs';
+import * as os from 'os';
+import * as path from 'path';
+import { afterEach, beforeEach, describe, expect, it, vi } from 'vitest';
+import {
+  handleRunAfterProductionCompile,
+  stripSourceMappingURLComments,
+} from '../../src/config/handleRunAfterProductionCompile';
 import type { SentryBuildOptions } from '../../src/config/types';
 
 vi.mock('@sentry/core', () => ({
@@ -305,6 +311,85 @@ describe('handleRunAfterProductionCompile', () => {
     });
   });
 
+  describe('sourceMappingURL stripping', () => {
+    let readdirSpy: ReturnType<typeof vi.spyOn>;
+
+    beforeEach(() => {
+      // Spy on fs.promises.readdir to detect whether stripping was attempted.
+      // The actual readdir will fail (dir doesn't exist), which is fine — we just
+      // need to know if it was called.
+      readdirSpy = vi.spyOn(fs.promises, 'readdir').mockRejectedValue(new Error('ENOENT'));
+    });
+
+    afterEach(() => {
+      readdirSpy.mockRestore();
+    });
+
+    it('strips sourceMappingURL comments for turbopack builds with deleteSourcemapsAfterUpload', async () => {
+      await handleRunAfterProductionCompile(
+        {
+          releaseName: 'test-release',
+          distDir: '/path/to/.next',
+          buildTool: 'turbopack',
+        },
+        {
+          ...mockSentryBuildOptions,
+          sourcemaps: { deleteSourcemapsAfterUpload: true },
+        },
+      );
+
+      expect(readdirSpy).toHaveBeenCalledWith(
+        path.join('/path/to/.next', 'static'),
+        expect.objectContaining({ recursive: true }),
+      );
+    });
+
+    it('does NOT strip sourceMappingURL comments for webpack builds even with deleteSourcemapsAfterUpload', async () => {
+      await handleRunAfterProductionCompile(
+        {
+          releaseName: 'test-release',
+          distDir: '/path/to/.next',
+          buildTool: 'webpack',
+        },
+        {
+          ...mockSentryBuildOptions,
+          sourcemaps: { deleteSourcemapsAfterUpload: true },
+        },
+      );
+
+      expect(readdirSpy).not.toHaveBeenCalled();
+    });
+
+    it('does NOT strip sourceMappingURL comments when deleteSourcemapsAfterUpload is false', async () => {
+      await handleRunAfterProductionCompile(
+        {
+          releaseName: 'test-release',
+          distDir: '/path/to/.next',
+          buildTool: 'turbopack',
+        },
+        {
+          ...mockSentryBuildOptions,
+          sourcemaps: { deleteSourcemapsAfterUpload: false },
+        },
+      );
+
+      expect(readdirSpy).not.toHaveBeenCalled();
+    });
+
+    it('does NOT strip sourceMappingURL comments when deleteSourcemapsAfterUpload is undefined', async () => {
+      await handleRunAfterProductionCompile(
+        {
+          releaseName: 'test-release',
+          distDir: '/path/to/.next',
+          buildTool: 'turbopack',
+        },
+        mockSentryBuildOptions,
+      );
+
+      expect(readdirSpy).not.toHaveBeenCalled();
+    });
+  });
+
   describe('path handling', () => {
     it('correctly passes distDir to debug ID injection', async () => {
       const customDistDir = '/custom/dist/path';
@@ -343,3 +428,112 @@ describe('handleRunAfterProductionCompile', () => {
     });
   });
 });
+
+describe('stripSourceMappingURLComments', () => {
+  let tmpDir: string;
+
+  beforeEach(async () => {
+    tmpDir = await fs.promises.mkdtemp(path.join(os.tmpdir(), 'sentry-test-'));
+    await fs.promises.mkdir(path.join(tmpDir, 'chunks'), { recursive: true });
+  });
+
+  afterEach(async () => {
+    await fs.promises.rm(tmpDir, { recursive: true, force: true });
+  });
+
+  it('strips sourceMappingURL comment from JS files', async () => {
+    const filePath = path.join(tmpDir, 'chunks', 'abc123.js');
+    await fs.promises.writeFile(filePath, 'console.log("hello");\n//# sourceMappingURL=abc123.js.map');
+
+    await stripSourceMappingURLComments(tmpDir);
+
+    const content = await fs.promises.readFile(filePath, 'utf-8');
+    expect(content).toBe('console.log("hello");');
+    expect(content).not.toContain('sourceMappingURL');
+  });
+
+  it('strips sourceMappingURL comment from MJS files', async () => {
+    const filePath = path.join(tmpDir, 'chunks', 'module.mjs');
+    await fs.promises.writeFile(filePath, 'export default 42;\n//# sourceMappingURL=module.mjs.map');
+
+    await stripSourceMappingURLComments(tmpDir);
+
+    const content = await fs.promises.readFile(filePath, 'utf-8');
+    expect(content).toBe('export default 42;');
+  });
+
+  it('strips sourceMappingURL comment from CSS files', async () => {
+    const filePath = path.join(tmpDir, 'chunks', 'styles.css');
+    await fs.promises.writeFile(filePath, '.foo { color: red; }\n/*# sourceMappingURL=styles.css.map */');
+
+    await stripSourceMappingURLComments(tmpDir);
+
+    const content = await fs.promises.readFile(filePath, 'utf-8');
+    expect(content).toBe('.foo { color: red; }');
+  });
+
+  it('does not modify files without sourceMappingURL comments', async () => {
+    const filePath = path.join(tmpDir, 'chunks', 'clean.js');
+    const originalContent = 'console.log("no source map ref");';
+    await fs.promises.writeFile(filePath, originalContent);
+
+    await stripSourceMappingURLComments(tmpDir);
+
+    const content = await fs.promises.readFile(filePath, 'utf-8');
+    expect(content).toBe(originalContent);
+  });
+
+  it('handles files in nested subdirectories', async () => {
+    const nestedDir = path.join(tmpDir, 'chunks', 'app', 'page');
+    await fs.promises.mkdir(nestedDir, { recursive: true });
+    const filePath = path.join(nestedDir, 'layout.js');
+    await fs.promises.writeFile(filePath, 'var x = 1;\n//# sourceMappingURL=layout.js.map');
+
+    await stripSourceMappingURLComments(tmpDir);
+
+    const content = await fs.promises.readFile(filePath, 'utf-8');
+    expect(content).toBe('var x = 1;');
+  });
+
+  it('handles non-existent directory gracefully', async () => {
+    await expect(stripSourceMappingURLComments('/nonexistent/path')).resolves.toBeUndefined();
+  });
+
+  it('handles sourceMappingURL with @-style comment', async () => {
+    const filePath = path.join(tmpDir, 'chunks', 'legacy.js');
+    await fs.promises.writeFile(filePath, 'var y = 2;\n//@ sourceMappingURL=legacy.js.map');
+
+    await stripSourceMappingURLComments(tmpDir);
+
+    const content = await fs.promises.readFile(filePath, 'utf-8');
+    expect(content).toBe('var y = 2;');
+  });
+
+  it('ignores non-JS/CSS files', async () => {
+    const filePath = path.join(tmpDir, 'chunks', 'data.json');
+    const originalContent = '{"key": "value"}\n//# sourceMappingURL=data.json.map';
+    await fs.promises.writeFile(filePath, originalContent);
+
+    await stripSourceMappingURLComments(tmpDir);
+
+    const content = await fs.promises.readFile(filePath, 'utf-8');
+    expect(content).toBe(originalContent);
+  });
+
+  it('processes multiple files concurrently', async () => {
+    const files = ['a.js', 'b.mjs', 'c.cjs', 'd.css'];
+    for (const file of files) {
+      const ext = path.extname(file);
+      const comment = ext === '.css' ? `/*# sourceMappingURL=${file}.map */` : `//# sourceMappingURL=${file}.map`;
+      await fs.promises.writeFile(path.join(tmpDir, file), `content_${file}\n${comment}`);
+    }
+
+    await stripSourceMappingURLComments(tmpDir);
+
+    for (const file of files) {
+      const content = await fs.promises.readFile(path.join(tmpDir, file), 'utf-8');
+      expect(content).toBe(`content_${file}`);
+      expect(content).not.toContain('sourceMappingURL');
+    }
+  });
+});